Polymorphisms within Intron-4 and Exon-4 regions of SPP1 gene and their association with milk traits in Gaolao cattle

Secreted phosphoprotein 1 (SPP1) is an acidic, phosphorylated glycoprotein found in fluids including milk and involved development of mammary gland exhibits impact on composition yield. The aim study was to reveal SPP1 gene polymorphisms association with traits Gaolao cattle. Blood collected from 280 cows test day recorded. SPP1G1-BsrI locus intron-IV polymorphic frequency TT genotype 0.99 CT 0.01, respectively. polymorphism revealed significant difference Milk yield SNF% for as compared genotype. Sequencing locus-based genotypes G-A SNP at 32nd position. SPP1G5-SSCP exon-4 region A pattern 0.95 B patterns 0.05. study, differences Lactose% B. Direct sequencing SSCP SNPs A-G 71st, A-C 109th 208th position 02 computational SNPs, viz. C-A Identified needs validation large data sets records markers bovine traits.